21PHY335 BIOPHYSICS-

UNIT I-

Introduction: Laws of Physics and Chemistry, introduction to crystallography, Introduction to chromatography, electrophoresis Physico-Chemical Techniques to study Biomolecules: hydration of macromolecules, diffusion of osmosis, sedimentation, ultracentrifuge, rotational dissuasion, light scattering, small angle X-ray scattering, Mass spectrometry.

UNIT II-

Spectroscopy: UV spectroscopy, circular dichroism, Fluorescence spectroscopy, IR, Raman and Electron spin spectroscopy, NMR spectroscopy.

UNIT III-

Molecular Modeling & Macromolecular Structure: building the structure of H2O2, nucleic-acid structure, monomers, polymers, double helical structure of DNA, Polymorphism and nanostructure of DNA, structure of RNA, protein structure: amino acids, virus structure.

UNIT IV-

Energy Pathways in Biology: free energy, couple reactions, group transfer potential, Pyridine Nucleotide, photosynthesis, energy conversion pathways, membrane transport, Biomechanics: strained muscles, mechanical properties of muscles, cardiovascular system.

UNIT V-

Neurophysics: nervous system, physics of membrane potentials, sensory mechanisms, Origin and evolution of life: prebiotic earth, theories of origin and evolution of life, laboratory experiments on formation of small molecules.

TEXTBOOKS:

1. “Cell and Molecular Biology-Concepts and Experiments” by G.Karp, 2nd ed. John Wiley & Sons, Inc. Singapore, 1999.
2. “Principles of Physical Biochemistry” by K.vanHolde, W.C. Johnson, and P.S.Ho. Prentice Hall, 1998.