BPI -102 Biophysics & Instrumentation-

General Biophysical methods- Measurement of pH, Radioactive labeling & counting, Autoradiography.

Separation & Identification of Materials- concept of Chromatography (Partition Chromatography, Paper Chromatography, Adsorption Chromatography, TLC, GLC, Ion Exchange Chromatography, Gel Chromatography, HPLC, Affinity Chromatography); Electrophoresis (Gel Electrophoresis, Paper Electrophoresis).

Centrifugation- Basic Principle of Centrifugation, Instrumentation of Ultracentrifuge (Preparative, Analytical), Factors affecting Sedimentation velocity, Standard Sedimentation Coefficient, Centrifugation of associating systems, Rate-Zonal centrifugation, sedimentation equilibrium Centrifugation.

Microscopy- Light microscopy, Bright & Dark Field microscopy, Fluorescence microscopy, Phase Contrast microscopy, TEM, SEM.

X-Ray Crystallography- X-ray diffraction, Bragg equation, Reciprocal lattice, Miller indices & Unit cell, Concept of different crystal structure, determination of crystal structure [concept of rotating crystal method, powder method].

Spectroscopy- Raman Spectroscopy – What is Raman effect, Quantum mechanical reason of Raman effect, Molecular Polarizability, Polarizability ellipsoid, Experimental technique of Raman effect, Basic concept of Pure Rotational & Vibrational, Raman spectra of simple molecule (linear molecule), NMR Spectroscopy – Basic principle of NMR spectroscopy, Experimental technique & instrumentation, Chemical shift, Hyperfine splitting, Relaxation process, Absorption Spectroscopy – Simple theory of the absorption of light by molecules, Beer-Lambert law, Instrumentation for measuring the absorbance of visible light, Factors affecting the absorption properties of a Chromophore.