PAT-303 Plant and Animal Tissue Culture Techniques and its application-

Introduction to Techniques- Introductory history, Laboratory organization, Media, Aseptic manipulation.

Basic concepts in cell culture- cell culture, Cellular Totipotency, Somatic Embryogenesis.

In vitro culture- approaches & methodologies – preparation steps for tissue culture, surface sterilization of plant tissue material, basic procedure for aseptic tissue transfer, incubation of culture.

Tissue nutrition- Growth Hormones – Plant cells (Composition of culture media, Growth hormones, Vitamins, Unidentified supplements, selection of media); Animal cells (substrate on which cells grow, Feeder layer on substrate, gas phase for tissue culture, media and supplements).

Tissue culture methodologies- Plant cells (Callus Culture, Cell Suspension Culture, Organ Microculture, plant micro-propagation, Somatic Embryogenesis); Animal cells (Source of tissue, primary culture, differentiation of cells, growth kinetics, animal cell lines and their origin and
chracterization).

Cloning & Selection of specific cell types- cloning, somatic cell fusion and HAT selection, Medium suspension fusion, selection of Hybrid clone, production of monoclonal antibodies.

Organ Culture- Culture of embryonic organs, whole embryo culture, culture of adult organs.