TCT-306 Tissue Culture Techniques-

1. In vitro Culture- Washing & Sterilization, Preparatory steps for tissue culture, surface sterilization of plant material, basic procedures for Aseptic tissue transfer, incubation of culture.
2. Preparation of Culture media & Reagents- Media composition, Nutrition, Hormones.
3. Tissue Culture- Callus culture, Cell suspension.
4. Organ Micro-culture- Shoot tip, excised root, Leaf culture.
5. Plant micro-propagation– micro-culture of plants.