MOG-401 Molecular Genetics-

How to clone a gene- What is clone, Overview of the procedure, Gene library, Hybridization.

Purification and Separation of nucleic acids- Extraction and Purification of nucleic acids, Detection and Quantitation of Nucleic acids, Gel Electrophoresis.

Cutting and Joining DNA- Restriction Endonucleases, Ligation, Alkaline Phosphate, Double Digest, Modification of Restriction Fragments ends, Other Ways of joining DNA Molecules.

Vectors- Plasmid vectors, Vectors based on the lambda Bacteriophage, Cosmids, M13 vectors, Expression vectors, Vectors for cloning and expression in Eukaryotic cells, Super vectors: YACs and BACs.

Amplifying DNA- PCR and Cell based DNA Cloning – The importance of DNA Cloning, PCR: basic features and application, Principles of Cell-based DNA Cloning, Cloning System for amplifying different sized fragments, Cloning System for producing single-stranded and mutagenized DNA.

Nucleic Acid Hybridization- Principle and application – Preparation of nucleic probes, Principle of Nucleic acid hybridization, Nucleic acid hybridization assays, and microarrays.