RDT-502 Recombinant DNA Technology-

Gene Recombination and Gene transfer- Bacterial Conjugation, Transformation, Transduction, Episomes, Plasmids, Microinjection, Electroporation, Microprojectile, Shot Gun method, Ultrasonication, Liposome fusion, Microlaser.

Changing genes- site-directed mutagenesis and Protein engineering: Primer extension is a simple method for site directed mutation, PCR based site directed mutagenesis, Random mutagenesis, Use of Phage display techniques to facilitate the selection of mutant peptides, Gene shuffling, production of chimeric proteins.

Genetic engineering in animals- Production of transgenic mice, ES cells can be used for gene targeting in mice, Applications of gene targeting, Using Yeast to study Eukaryotic gene function, Therapeutic products produced by genetic engineering-blood proteins, human hormones, immune modulators and vaccines, Transgenic animals, Production of proteins of Pharmaceutical value.

Genetic engineering in plants- Use of Agrobacterium tumefaciens and Arhizogenes, Ti plasmids, Strategies for gene transfer to plant cells, Direct DNA transfer to plants, Gene targeting in plants, Use of plant viruses as episomal expression vectors.