MSA –101 Macromolecular Structure & Analysis-
Carbohydrates: Structural aspects – Introduction & Occurrence, Classification of Mono, Di and Polysaccharides, Reducing & Non-reducing Sugars, Constitution of Glucose & Fructose, Osazone formation, Pyranose & Furanose forms, Determination of ring size, Inter-conversion of monosaccharides.
Lipids: Structural aspects – General introduction, Classification & Structure of Simple & Compound lipids, Properties of Lipid aggregates (elementary idea), Biological membrane, Membrane protein – structural aspects, Lipoproteins (elementary idea).
Proteins: Structural aspects – General introduction, Classification & General characteristics, Structure of Primary, Secondary, Tertiary & Quaternary proteins (elementary idea), chains of proteins (elementary idea), Classification of Amino acids.
Nucleic acid: Structural aspects – Components of DNA and RNA, Nucleosides & Nucleotides (introduction, structure & bonding), Double helical structure of DNA (Watson-Crick model), various forms of DNA.
Chemical & Enzymatic Kinetics: An introduction to enzyme; How enzyme works; Reaction rate; Thermodynamic definitions; Principles of catalytic power and specificity of enzymes; Enzyme kinetics – Approach to mechanism.
Genes are DNA: DNA is the genetic material, DNA is a double helix, DNA replication is semiconservative, mutations change the sequence of DNA, a gene codes for a single polypeptide, recombination occurs by physical exchange of DNA, genetic code is triplet.
Mutation: Occurrence, kinds of Mutation, spontaneous & induced Mutation, Mutagens, detection of Mutation, Lethal Mutations, Biochemical Mutations, Phenotypic effects of Mutation, Molecular basis of Mutation, Significance & Practical applications of Mutation.
Expression of genetic information: from Transcription to Translation – The Relationship between genes and protein, The transcriptions: The basic process, Transcription and RNA Processing in Eukaryotic Cells, Encoding genetic information, Decoding the codons: the role of transfer RNAs.
Regulation of mRNA stability: capping, polyadenylation, pre-mRNA splicing, formation of commitment complex, creation of catalytic sites, trans-esterification reactions, mRNA surveillance.