BIOL-310 Techniques and Instrumentations in Biology-
UNIT I-
Principles and handling of Microscope, Spectrophotometer, Colorimeter, Flame photometer, Autoclave, Laminar air flow, Fermenter (Bioreactor), Electronic and Electrical balance, Refrigerator, minor Instrument and Glass wares.
UNIT II-
Principles, types working and maintenance of Microscopes, centrifuge, incubator, colorimeter, Spectrophotometer, Electrophoresis and Chromatography.
UNIT III-
Sterilization techniques, Preparation of different types of solutions, culture techniques & cryopreservation.
UNIT IV-
Herbaria Techniques, Microtomy staining techniques, Preparation of permanent mount, specimen preservation techniques.
UNIT V-
Seed viability testing, testing of pollen viability, Tissue culture of crop plants; description of flowering plants in botanical terms in relation to taxonomy.
UNIT VI-
Preparation of different agrochemical doses in field and pot application.
PRACTICALS:
- Study of different parts of simple and compound microscopes
- Preparation of Solutions and buffers
- Study of different parts of centrifuge and types of rotors
- Study of electrophorectic apparatus