BIOL-310 Techniques and Instrumentations in Biology-

UNIT I-

Principles and handling of Microscope, Spectrophotometer, Colorimeter, Flame photometer, Autoclave, Laminar air flow, Fermenter (Bioreactor), Electronic and Electrical balance, Refrigerator, minor Instrument and Glass wares.

UNIT II-

Principles, types working and maintenance of Microscopes, centrifuge, incubator, colorimeter, Spectrophotometer, Electrophoresis and Chromatography.

UNIT III-

Sterilization techniques, Preparation of different types of solutions, culture techniques & cryopreservation.

UNIT IV-

Herbaria Techniques, Microtomy staining techniques, Preparation of permanent mount, specimen preservation techniques.

UNIT V-

Seed viability testing, testing of pollen viability, Tissue culture of crop plants; description of flowering plants in botanical terms in relation to taxonomy.

UNIT VI-

Preparation of different agrochemical doses in field and pot application.

PRACTICALS:

  1. Study of different parts of simple and compound microscopes
  2. Preparation of Solutions and buffers
  3. Study of different parts of centrifuge and types of rotors
  4. Study of electrophorectic apparatus