MCE-302 Molecular biology-
UNIT I-
Basic introduction to molecular biology, Structural aspects of nucleic acids and proteins.
UNIT II-
Replication and transcription in eukaryotes and prokaryotes; regulation and post transcriptional modification; concept of lac & trp operon.
UNIT III-
Concept of genetic codon and modern concept of gene.
UNIT IV-
Translation and post translational modification.
UNIT V-
Transposons and extra nuclear inheritance.
Practical:
Basic methodology to molecular biology
Preparation of reagent
Protein isolation and quantization
DNA isolation and agarose gel electrophoresis
DNA purification