MCE-302 Molecular biology-

UNIT I-

Basic introduction to molecular biology, Structural aspects of nucleic acids and proteins.

UNIT II-

Replication and transcription in eukaryotes and prokaryotes; regulation and post transcriptional modification; concept of lac & trp operon.

UNIT III-

Concept of genetic codon and modern concept of gene.

UNIT IV-

Translation and post translational modification.

UNIT V-

Transposons and extra nuclear inheritance.

Practical:

 Basic methodology to molecular biology
 Preparation of reagent
 Protein isolation and quantization
 DNA isolation and agarose gel electrophoresis
 DNA purification