CBBT-501 Applications in Bioinformatics-

Theory:

Bioinformatics and Internet: Internet Basics, FTP, www, connecting to Internet, Electronic mail, internet resources.

The NCBI data model: Introduction, Seq-id, Sequence, collection of sequence, annotation of sequence, describing sequence.

GenBank Sequence Database: Introduction to structure, Primary and secondary database.

Sequence Alignment And Database Searching: FASTA, BLAST.

Multiple Sequence Alignment:

Phylogenetic Analysis: Fundamental of Phylogenetic model, Tree interpretation, Tree building and tree evaluation, Phylogenetic software.

Introduction to Structure: PDB, MMDB, Structure file format, visualizing structure information, Structure viewers, structure similarity searching, Advanced structure modeling.

Comparative Genome Analysis: Introduction, application, genome analysis and annotation.

Practicals:

  1. Understanding Linux Operating System and Commands.
  2. Introduction to NCBI.
  3. Using Entrez to search Literature Databases.
  4. Retrieving DNA sequence from GenBank and analyzing various formats of the data stored.
  5. Retrieving Protein sequence from GenPept (NCBI) and Expasy.
  6. Analyzing Protein Sequences.
  7. Analyzing DNA sequence.
  8. Sequence alignment using BLAST (Basic Local Alignment Search Tool).
  9. Sequence alignment using FASTA.
  10. Multiple sequence alignment using ClustalW.