CBBT-501 Applications in Bioinformatics-
Theory:
Bioinformatics and Internet: Internet Basics, FTP, www, connecting to Internet, Electronic mail, internet resources.
The NCBI data model: Introduction, Seq-id, Sequence, collection of sequence, annotation of sequence, describing sequence.
GenBank Sequence Database: Introduction to structure, Primary and secondary database.
Sequence Alignment And Database Searching: FASTA, BLAST.
Multiple Sequence Alignment:
Phylogenetic Analysis: Fundamental of Phylogenetic model, Tree interpretation, Tree building and tree evaluation, Phylogenetic software.
Introduction to Structure: PDB, MMDB, Structure file format, visualizing structure information, Structure viewers, structure similarity searching, Advanced structure modeling.
Comparative Genome Analysis: Introduction, application, genome analysis and annotation.
Practicals:
- Understanding Linux Operating System and Commands.
- Introduction to NCBI.
- Using Entrez to search Literature Databases.
- Retrieving DNA sequence from GenBank and analyzing various formats of the data stored.
- Retrieving Protein sequence from GenPept (NCBI) and Expasy.
- Analyzing Protein Sequences.
- Analyzing DNA sequence.
- Sequence alignment using BLAST (Basic Local Alignment Search Tool).
- Sequence alignment using FASTA.
- Multiple sequence alignment using ClustalW.